Plant reproduction represents a key stage on plant development, providing fruits and seeds that sustain global food supply. With rising global temperatures this stage is severely threatened, affecting fertilization efficiency, crop yield and food security. Upon pollination, most pollen tube (PT) growth occurs along a dynamic signaling interface within the pistil: the transmitting tract (TT). TT cells provide structural support, nutritional resources, adhesion cues, and directional guidance through a highly specialized extracellular matrix (ECM). Although PT-pistil interactions are extensively studied, a significant gap remains on TT’s contribution, especially under heat-stress (HS). Questions about the identity of extracellular signaling molecules, their receptors, and downstream pathways governing TT ECM remodeling and cell wall dynamics are still unanswered. Thus, we aim to shift the focus from the male-centric paradigms to female tissue determinants of fertilization by combining transcriptomics, cell biology, and advanced imaging in Arabidopsis thaliana and Solanum lycopersicum cv MicroTom, as a translational tool for crop species. We are analyzing bulk RNA-seq data of Arabidopsis pistils in which TT function is impaired, under control and HS conditions and processing immunocytochemistry samples that will allow us to perceive morphological changes in the pistil within these conditions.