Plant Polysaccharide Antibody Database (PPAD) – an interactive online resource for information about antibody specificities and epitope structures
Ieva Lelėnaitė (UK)1; Jonatan Fangel (Denmark)2 3; Charlotte Stenkjær Fletcher (UK)1; Cassie Bakshani (UK)4; Catherine Tétard-Jones (UK)5; Stjepan Kresimir Kracun (Denmark)6; Steven Hall (UK)1; Peter Ulvskov (Denmark)7; William G.T. Willats (UK)1;
1 - Newcastle University; 2 - Novozymes; 3 - Novonesis; 4 - University of Birmingham; 5 - Leica Biosystems Newcastle Ltd; 6 - Christian Hansen A/S; 7 - University of Copenhagen;
Keywords: Polysaccharides; Antibodies; Database;
Abstract Topics: Theme 10: Tools, Imaging, and Omics for Cell Wall Research
Type of Presentation: Poster

Abstract text: Glycan-directed molecular probes including monoclonal antibodies (mAbs) are powerful and widely used tools for cell wall analysis. The number of mAbs has steadily grown, as has the degree of detail regarding their specificities. This makes it increasingly difficult for researchers to easily assimilate the information required to make choices regarding mAb usage, and to interpret outputs. Currently, information about cell wall mAbs is distributed across multiple publications and some websites. We have developed the Plant Polysaccharide Antibody Database (PPAD) that greatly simplifies the process of finding what mAbs are available and what they bind to. PPAD is an open-access web-based interactive search tool enabling user-friendly interrogation of a large underlying data set generated by screening 189 mAbs against 56 polysaccharides, 59 oligosaccharides and 60 extractions from diverse plant species. mAb screening was performed using high-density microarrays, allowing for large numbers of replicates. However, the database is designed such that additional datasets (microarray, ELISA or other types) may be included in the future.