Abstract text: Extensins (EXTs) are diverse hydroxyproline-rich glycoproteins (HRGPs) found in the cell wall that exert their functions through self-assembly and cross-linking. These structural proteins are thought to act as molecular scaffolds on which various biomolecules, like pectin, attach to form extensin networks deposited in the growing cell wall. Here, we utilized a synthetic gene approach to design and construct extensin analogs. These analogs will be used to understand the self-assembly of EXTs and to elicit the role of amphiphilicity in cell wall assembly. The extensin analogs containing repetitive motifs of [Ser-Hyp-Hyp-Hyp-Hyp-Tyr-Try-Tyr-Lys]_n, were synthesized using a modified cloning technique. Varying lengths of these extensin analogs were prepared from a single repeat. The extensin analogs were expressed transiently in Nicotiana tabacum leaves, and cellular localization was confirmed in the cell wall. Stable mutant lines harboring the extensin fusion protein analogs were prepared. The proteins will undergo biochemical and biophysical characterizations. These studies may contribute to the molecular-level understanding of the plant cell wall assembly, architecture, and function. Moreover, these findings may be useful in the design of new biopolymers and biomaterials for varied medical, industrial, and pharmaceutical applications.