Abstract text: The leaf outermost epidermis cell layer of the leaf sense and responds to environmental threat, protecting the plants against various stresses such as wind, rain drought and pests. The epidermis cell layers sandwiches the porous, low-density mesophyll cells layers specifically, palisade and spongy mesophylls, which maintain tissue integrity and contribute to the mechanical stiffness to the leaf. Epidermis cells in the leaf exhibit diverse shapes and include specialized structures such as trichomes, stomatal complexes, and pavement cells, specialized for different functions and developmentally coordinated. During the expansion of epidermal pavement cell, the elongation of one cell inevitably leads to the indentation of the neighboring one(s). Thus, pavement cells display an interlocking jigsaw-puzzle pattern with multiple protrusion and indentation that interdigitate among their neighboring pavement cells. The diverse physical and mechanochemical nature of pavement cell wall is crucial for epidermal cell-shape acquisition to maintain the leaf tissue integrity. However, the contribution of mesophyll sublayers in regulating the shape and tissue integrity of epidermal pavement cells remain poorly understood. To address this knowledge gap, we screened several mesophyll cell mutants with defects in cell shape and density in Arabidopsis leaves and analysed the resulting shapes of epidermal pavement cells. Additionally, we mechanically ablate the single palisade mesophyll cells and followed the pavement cells shape at different time points using live cell imaging. Our analysis indicates that mesophyll sublayer certainly participates to the shape of epidermal pavement cells through mechanical and/or molecular signaling mechanisms.