Abstract text: Plant cell walls exhibit a hierarchical architecture in which cellulose microfibril orientation governs mechanical performance and biological function. In wood, this organization spans multiple length scales, yet quantitative three-dimensional descriptions across the scales remain limited. Here, we investigate cellulose fibril orientation in Norway spruce wood by combining electron tomography and microbeam small-angle X-ray scattering (SAXS) with three-dimensional reconstruction approaches. Electron tomography provides nanoscale 3D reconstructions of microfibril organization within localized cell wall regions, enabling characterization of local orientation distributions. SAXS data measured with microscale spatial resolution (~50 µm) are analyzed through azimuthal intensity distributions and the results are reconstructed into a 3D map describing the orientation distributions. Orientation metrics, including peak positions and angular widths, are compared across length scales to assess spatial heterogeneity and scale-dependent averaging effects. The results reveal differences between locally resolved and microscale reconstructed fibril orientations, highlighting how hierarchical organization in wood cell walls influences commonly used orientation descriptors like the microfibril angle. By integrating real-space and reciprocal-space three-dimensional reconstructions, this work advances a multiscale framework for describing cellulose organization in plant secondary cell walls and provides new insight into structure-function relationships in wood.