Abstract text: A key property of all multicellular systems is intercellular communication which coordinates growth and responses to external cues. Plasmodesmata connect neighbouring cells enabling the transport of small and large molecules including signalling proteins and RNAs, metabolites and hormones. Our lab studies the composition and properties of cell walls surrounding plasmodesmata. The metabolism of β-1,3 glucan (i.e, callose) regulates plasmodesmata aperture and connectivity. Using new monoclonal antibodies, we revealed callose structural diversity and glycan interactions underpinning its function in plasmodesmata regulation. We build on this knowledge and identify genetic targets to modify the properties of cell walls surrounding plasmodesmata as a route to improve developmental and environmental signalling. Using these tools, I will report how we modify root growth under osmotic stress conditions and the establishment of symbiotic structures tackling environmental and sustainability challenges.