Abstract text: Plant cell walls are polysaccharide-rich extracellular matrices that contain multiple glycan networks, including cellulose, hemicelluloses, pectins, and glycosylated proteins. The proper deposition of these polysaccharides is critical for wall structure, and structural microheterogeneity within these glycans can influence polymer rigidity or polymer-polymer interactions. Three large collections of monoclonal antibodies (mAbs) have been developed to target unique epitopes within common cell wall polysaccharides to investigate how these structural changes impact cellular and plant development, but their implementation in high-throughput contexts has been challenging. Here, we develop methods to attach unique DNA barcodes to a subset of mAbs that recognize major cell wall polysaccharide classes. We demonstrate that these mAbs can be applied individually to polysaccharide standards, and that the abundance of bound DNA barcoded antibody can be measured via qPCR. Additionally, we demonstrate that these DNA barcoded antibodies can be pooled to probe polysaccharide standards and fractionated cell wall material to yield quantitative insights through the amplification of their unique barcodes. Currently, these results indicate that this strategy facilitates multiplexed profiling of cell wall polysaccharide structure and abundance. Future directions of this approach and efforts to sustain major cell wall mAb collections will also be discussed.