Abstract text: Plant cell walls (CW) exhibit architectural diversity; however, most studies focus on model species or major crops. Native Chilean fruits represent an underexplored source of CW variation. This is exemplified by Chilean papaya seed mucilage, where highly methylesterified homogalacturonan is its main component. Extending this approach to other native fruits, Aristotelia chilensis (maqui) and Berberis microphylla (calafate) are Patagonian berries recognised for their high antioxidant capacity, yet their CW polysaccharides remain uncharacterised. We investigate the CW composition of maqui and calafate. Maqui alcohol-insoluble residues (AIR) are enriched in xylose and glucose, indicating abundant hemicelluloses, likely xylans and xyloglucans. Conversely, calafate AIR is rich in arabinose, with notable amounts of galactose, mannose, xylose, and galacturonic acid. Its hemicelluloses comprise xylose-, arabinose-, glucose-, galactose-, and mannose-containing polymers, indicative of mixed xylan- and mannan-based networks. In pectin-enriched fractions from both berries, homogalacturonan predominates over RG-I, which appears highly branched with arabinan and galactan side chains, alongside the presence of AGPs. Preliminary assays using pectin domains from papaya mucilage enhanced viability and proliferation in skin cell lines and murine macrophages, highlighting the biomedical potential of fruit pectins. These findings emphasise the value of exploring pectin diversity, structure–function relationships, and biotechnological applications.