Compared to animals, plant have evolved remarkable capacities to repair tissues and even regenerate new organs or whole plants upon injury. The ETHYLENE RESPONSE FACTOR 114 (ERF114) and ERF115 play a paramount role in this process enabling for example callus formation, de novo root regeneration and root meristem regeneration through the upregulation of described downstream targets. However, the upstream signals linking wounding to ERFs induction and regeneration are still unknown. Through the combination of metabolomic analysis, pharmacological and genetic approaches, we found that expression of the ERFs is linked with pectin modifications in Arabidopsis thaliana but also poplar. Pectin fragments called oligogalacturonides (OGs) can activate the immune response as damage associated molecular patterns but were never linked to a root developmental process such as regeneration. Here, we report for the first time that OGs carry a root regeneration promoting potential. OG treatment could induce expression of the ERFs and promote a faster root organogenesis in an ERF115-dependent manner. This positive effect was dependent on the size but not the esterification status of OGs. Lastly, we found that OGs act additively with auxin treatment in promoting root regeneration, revealing a promising tool to achieve regeneration in recalcitrant plant species.